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Purification of supercoiled plasmid PM by affinity capture. The initial DNA mixture comprised DNA with the perfectly matched target in supercoiled (sc PM) and linear form (lin PM), as well as two DNA fragments not containing any target sequence (NT1 and NT2). Conditions of PNA1 binding: (A) [PNA]: 1 µM, 1 h at 42°C with 60 mM NaCl added, (B) [PNA]: 1 µM, 3 h at 42°C with 50 mM NaCl added, (C) [PNA]: 3 µM, 3 h at 42°C with 30 mM NaCl added. M: 1 kb DNA ladder.
