Table 1.

Kinetics of dsDNA invasion by PNA1

[Na+]/mMa	Target	k/min−1	Target	k/min−1	kMatch/kMismatch
			SM-1	(1.45 ± 0.12)·10−2	6.5
15	PM	(9.46 ± 0.41)·10−2	SM-2	(2.61 ± 0.09)·10−2	3.6
			SM-3	(3.33 ± 0.23)·10−2	2.8
			SM-1	(6.40 ± 0.66)·10−3	7.8
25	PM	(4.96 ± 0.26)·10−2	SM-2	(1.11 ± 0.08)·10−2	4.5
			SM-3	(1.32 ± 0.09)·10−2	3.8
			SM-1	(1.29 ± 0.09)·10−3	18.1
35	PM	(2.34 ± 0.09)·10−2	SM-2	(2.75 ± 0.08)·10−3	8.5
			SM-3	(4.76 ± 0.42)·10−3	4.9
45	PM	(7.93 ± 0.59)·10−3
55	PM	(4.03 ± 0.19)·10−3

^aAll reactions were performed in triplicate at 42°C in Na-phosphate buffer solutions (pH 7.0) with the indicated total concentration of Na⁺.