Figure 5.

Complementation of an E. coli thyA mutant by ThyX of CMP1. Logarithmic cells of E. coli SE1 thyA pSCodon_thyX grown in TBY with thymine (50 µg/ml) were washed twice and resuspended in TBY with or without thymine. One half of both cultures was induced with 0.1 mM IPTG. O.D₆₃₀ was measured every hour. Cells grown in TBY without thymine (squares), with thymine (triangles), without IPTG (filled symbols), with IPTG (open symbols). The complementation assay was repeated three times with similar results.