Figure 1.

Lysis cassette of ϕKMV. (A) Gene cluster. The lysis cassette is located in the ‘late’ region of ϕKMV genes.¹²,¹⁴ Four predicted overlapping lysis genes encoding a holin (KMV44), an endolysin (KMV45), Rz (KMV46) and Rz1 (KMV46.1) are delineated on the ϕKMV genome (total length is 42,519 bp). The corresponding borders (in nucleotide positions) are marked. (B) The N-terminal signal-arrest-release domain of KMV45. The positively charged N-region and the hydrophobic H-region of KMV45 are indicated. The conserved catalytic residues of the catalytic domain are underlined.