Table 2.
RAPD Primers Used to Genotype the Mapping Population Derived from a Cross T. durum × T. dicoccoides
| Primer1 | Sequence2 | No. of alleles | Fragment size (bp) |
|---|---|---|---|
| OPB6 | TGCTCTGCCC | 2 | 540, 350 |
| OPT7 | GGCAGGCTGT | 2 | 570, 340 |
| OPT16 | GGTGAACGCT | 2 | 560, 300 |
| UBC181 | ATGACGACGG | 2 | 600, 520 |
| UBC199 | GCTCCCCCAC | 3 | 1000, 640, 430 |
| UBC212 | GCTGCGTGAC | 4 | 1100, 1260, 360, 300 |
| UBC237 | CGACCAGAGC | 3 | 620, 370, 330 |
| UBC277 | AGGAAGGTGC | 2 | 590, 410 |
| UBC318 | CGGAGAGCGA | 3 | 790, 550, 350 |
| UBC359 | AGGCAGACCT | 1 | 510 |
| UBC388 | CGGTCGCGTC | 2 | 530, 430 |
| UBC399 | TTGCTGGGCG | 5 | 860, 830, 650, 410, 390 |
| UBC620 | TTGCGCCCGG | 5 | 810, 560, 510, 350, 300 |
| UBC625 | CCGCTGGAGC | 3 | 870, 800, 600 |
| SCAR199 | Left GCTCCCCCACTTGACTATTCA | 1 | 700 |
| Right CGAAGCCCTATTGGAATCCC | |||
| Total | 40 |
1
SCAR199 is a sequence-tagged-site marker derived from the 700bp-RAPD band generated by UBC199 in Triticum dicoccoides selection G25; 2DNA sequences are given in the orientation of 5′ to 3′.