Figure 1.

(A) Schematic of gene cloning and transfer by recombinational cloning. The triangles represent recombination sites. Genes are cloned into Entry Vectors by in vitro recombination of PCR products or with restriction enzymes and ligase. Thereafter, genes are moved entirely by recombination. (B) Subcloning of a gene from the Entry Vector into a Destination Vector. (C) Cloning attB-PCR products by in vitro recombination. (D) Sequences of the attB1 and attB2 sites that flank the gene either in a PCR product made with primers containing attB sites or in an Expression Clone.