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. 2011 Jan 7;1:143. doi: 10.3389/fmicb.2010.00143

Table 3.

Proteins which are recognized by sera from convalescent humans or from LVS vaccinated mice.

Locus tag Protein name Gene Reactivity sera1 Screening method2 PSORT3 Reference
FTT_0018 Secretion protein Human Proteome microarray Cytoplasmic membrane Sundaresh et al. (2007)
FTT_0037 NADH dehydrogenase I G subunit Mouse 2D-Western blot Unknown Twine et al. (2010)
FTT_0049 N utilization substance protein A nusA Mouse 2D-Western blot Cytoplasmic Twine et al. (2006a)
FTT_0060 ATP synthase B chain atpF Mouse Proteome microarray Cytoplasmic Eyles et al. (2007)
FTT_0062 ATP synthase alpha chain atpA Mouse 2D-Western blot Unknown Twine et al. (2006b, 2010)
FTT_0064 ATP synthase beta chain Mouse 2D-Western blot Cytoplasmic Huntley et al. (2007), Twine et al. (2010)
FTT_0071 Citrate synthase gltA Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_0074 Succinate dehydrogenase catalytic and NAD flavoprotein subunit shdA Human, presumed type B; mouse 2D-Western blot Unknown Twine et al. (2006b), Janovska et al. (2007a)
FTT_0075 Succinate dehydrogenase iron-sulfur subunit sdhB Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_0077 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex sucB Human, type A; human, presumed type B; mouse Proteome microarray/2D-Western blot Cytoplasmic Twine et al. (2006b), Eyles et al. (2007), Janovska et al. (2007a,b), Sundaresh et al. (2007), Twine et al. (2010)
FTT_0083 Hypothetical membrane protein Human, presumed type B Proteome microarray Unknown Sundaresh et al. (2007)
FTT_0086 Hypothetical protein Mouse 2D-Western blot Unknown Twine et al. (2006b)
FTT_0087 Aconitate hydratase Human, type A; human, presumed type B; mouse 2D-Western blot Cytoplasmic Janovska et al. (2007a,b), Twine et al. (2010)
FTT_0101 Conserved membrane hypothetical protein Human, presumed type B; mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007), Sundaresh et al. (2007)
FTT_0106 Efflux protein, RND family, MFP subunit Human, presumed type B; mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007), Sundaresh et al. (2007)
FTT_0119 Hypothetical membrane protein Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Eyles et al. (2007), Sundaresh et al. (2007), Twine et al. (2006a)
FTT_0123 Pseudogene Human, presumed type B Proteome microarray Cyt mem Sundaresh et al. (2007)
FTT_0137 Elongation factor Tu tufA Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2002, 2005), Twine et al. (2006a,b, 2010), Janovska et al. (2007a)
FTT_0141 50S ribosomal protein L1 rplA Mouse 2D-Western Blot Unknown Twine et al. (2006b)
FTT_0143 50S ribosomal protein L7/L12 rplL Human, presumed type B; mouse 2D-Western blot Unknown Havlasova et al. (2002, 2005), Twine et al. (2006a,b), Janovska et al. (2007a)
FTT_0183c 30S ribosomal protein S1 rpsA Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b, 2010)
FTT_0188 Cell division protein ftsZ Human, presumed type B; mouse 2D-Western blot Cytoplasmic Twine et al. (2006b, 2010), Janovska et al. (2007a)
FTT_0189 UDP-3-O-[3-hydroxymyristoyl] Mouse 2D-Western Blot Unknown Twine et al. (2010)
FTT_0194 Conserved hypothetical membrane protein Human, presumed type B Proteome microarray Cyt mem Sundaresh et al. (2007)
FTT_0196c Glutamine synthetase glnA Mouse Proteome microarray Cytoplasmic Eyles et al. (2007)
FTT_0208c ABC transporter, ATP-binding protein Mouse 2D-Western blot Unknown Twine et al. (2006b)
FTT_0209c Periplasmic solute binding family protein Mouse 2D-Western blot Unknown Twine et al. (2006a,b, 2010)
FTT_0233 Inner-membrane protein yidC Mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007)
FTT_0280 Major facilitator superfamily (MFS) transport protein Human, presumed type B Proteome microarray Cyt mem Sundaresh et al. (2007)
FTT_0296 Pyrrolidone-carboxylate peptidase pcp Mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2005), Eyles et al. (2007)
FTT_0313 30S ribosomal protein S2 rpsB Mouse 2D-Western blot Unknown Twine et al. (2006b)
FTT_0314 Protein chain elongation factor EF-Ts tsf Mouse 2D-Western blot/proteome microarray Cytoplasmic Havlasova et al. (2005), Twine et al. (2006a,b), Eyles et al. (2007)
FTT_0323 Elongation factor G fusA Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b, 2010)
FTT_0342 30S ribosomal protein S5 rpsE Mouse 2D-Western blot Unknown Twine et al. (2006b)
FTT_0350 DNA-directed RNA polymerase rpoA1 Mouse 2D-Western blot Cytoplasmic Twine et al. (2006a, 2010)
FTT_0356 Heat shock protein htpG Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005), Twine et al. (2006b), Janovska et al. (2007a)
FTT_0373c Nucleoside diphosphate kinase ndk Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005), Twine et al. (2006b)
FTT_0385 Hypothetical protein Mouse Proteome microarray Unknown Eyles et al. (2007)
FTT_0394 Hypothetical protein Human, presumed type B 2D-Western blot Unknown Havlasova et al. (2002)
FTT_0407 Glycine cleavage complex protein T gcvT Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2002, 2005)
FTT_0448c Glutaminyl-tRNA synthetase glnS Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_0471 3-Dehydroquinate dehydratase aroD Human, presumed type B 2D-Western blot Cytoplasmic Havlasova et al. (2002)
FTT_0472 Acetyl-CoA carboxylase, biotin carboxyl carrier protein subunit accB Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2002), Twine et al. (2006b, 2010), Eyles et al. (2007), Janovska et al. (2007a), Sundaresh et al. (2007)
FTT_0473 Acetyl-CoA carboxylase, biotin carboxylase subunit accC Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_0479 PerM family Human, presumed type B Proteome microarray Cytoplasmic membrane Sundaresh et al. (2007)
FTT_0503 Succinyl-CoA synthetase sucD Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005), Twine et al. (2006b)
FTT_0504c Succinyl-CoA synthetase subunit beta Human type A 2D-Western blot Cytoplasmic Janovska et al. (2007b)
FTT_0510 DNA gyrase subunit B Mouse 2D-Western blot Cytoplasmic Twine et al. (2010)
FTT_0511 Pyridoxine/pyridoxal 5-phosphate biosynthesis protein Mouse 2D-Western blot Cytoplasmic Twine et al. (2006a, 2010)
FTT_0535c Malate dehydrogenase mdh Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_0557 lemA-like protein Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_0580 Hypothetical protein Mouse 2D-Western blot Cytoplasmic Twine et al. (2010)
FTT_0583 Outer membrane associated protein fopA Human, presumed type B; mouse 2D-Western blot/proteome microarray Outer membrane Havlasova et al. (2005), Twine et al. (2006a,b, 2010), Eyles et al. (2007), Huntley et al. (2007), Janovska et al. (2007a)
FTT_0614 Apolipoprotein N-acyltransferase Human, presumed type B Proteome microarray Cytoplasmic mem Sundaresh et al. (2007)
FTT_0627 DNA binding protein hupB Human, presumed type B 2D-Western blot Unknown Havlasova et al. (2002), Janovska et al. (2007a)
FTT_0630 Host factor I for bacteriophage Q beta replication hfq Mouse 2D-Western blot/proteome microarray Cytoplasmic Havlasova et al. (2005), Eyles et al. (2007)
FTT_0682 Hypothetical protein Human, presumed type B; mouse Proteome microarray Periplasmic Eyles et al. (2007), Sundaresh et al. (2007)
FTT_0708 Major facilitator superfamily (MFS) transport protein Human, presumed type B Proteome microarray Cytoplasmic membrane Sundaresh et al. (2007)
FTT_0715 Chitinase family protein – inner membrane Mouse 2D-Western blot Unknown Havlasova et al. (2005), Twine et al. (2006b, 2010)
FTT_0721c Peroxidase/catalase katG Human type A; human, presumed type B; mouse 2D-Western blot Periplasmic space/outer membrane Havlasova et al. (2005), Twine et al. (2006a,b, 2010), Huntley et al. (2007), Janovska et al. (2007a,b
FTT_0724 Part of pseudogene dacB1 Mouse Proteome microarray Eyles et al. (2007)
FTT_0726c Glycerophosphoryl diester phosphodiesterase family protein Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_0756 Cation-efflux family protein Mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007)
FTT_0817 Threonyl-tRNA synthetase thrS Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_0831c OmpA family protein Human, presumed type B; mouse Proteome microarray/2D-Western blot Unknown Eyles et al. (2007), Huntley et al. (2007), Janovska et al. (2007a), Twine et al. (2010)
FTT_0849 Bile acid symporter family protein Human, presumed type B Proteome microarray Cytoplasmic mem Sundaresh et al. (2007)
FTT_0863 LemA-like protein Human, presumed type B; mouse 2D-Western blot/proteome microarray Cytoplasmic Twine et al. (2006b, 2010), Eyles et al. (2007), Janovska et al. (2007a), Sundaresh et al. (2007)
FTT_0869 Hypothetical protein Human, presumed type B; mouse Proteome microarray Unknown Eyles et al. (2007), Sundaresh et al. (2007)
FTT_0901 Conserved hypothetical lipoprotein (17 kDa major membrane protein precursor) lpnA Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2002), Eyles et al. (2007), Huntley et al. (2007), Janovska et al. (2007a)
FTT_0918 Hypothetical protein Human type A; mouse 2D-Western blot Outer membrane Twine et al. (2006a, Janovska et al. (2007b)
FTT_0949 Pseudogene Human, presumed type B Proteome microarray Unknown Sundaresh et al. (2007)
FTT_0956c Hypothetical membrane protein Human, presumed type B; mouse Proteome microarray Unknown Eyles et al. (2007), Sundaresh et al. (2007)
FTT_0975 Hypothetical protein Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2005), Eyles et al. (2007), Sundaresh et al. (2007)
FTT_0989 Hypothetical protein Human, presumed type B Proteome microarray Unknown Sundaresh et al. (2007)
FTT_0991 Hypothetical lipoprotein Human, presumed type B Proteome microarray Unknown Sundaresh et al. (2007)
FTT_1043 FKBP-type peptidyl-prolyl cistrans isomerase family protein Mouse 2D-Western blot Outer Membrane Twine et al. (2006b)
FTT_1060c 50S ribosomal protein L9 rplI Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2002, 2005), Twine et al. (2006b, 2010)
FTT_1097 Hypothetical protein Mouse Proteome microarray Unknown Eyles et al. (2007)
FTT_1103 Conserved hypothetical lipoprotein Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Twine et al. (2006b, 2010), Eyles et al. (2007), Janovska et al. (2007a)
FTT_1115 Preprotein translocase, subunit D, membrane protein secD Mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007)
FTT_1116 Preprotein translocase family protein yajC Human, presumed type B; mouse Proteome microarray Unknown Eyles et al. (2007), Sundaresh et al. (2007)
FTT_1125 d-methionine binding transport protein, ABC transporter, membrane and periplasmic protein metIQ Mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007)
FTT_1156c Type IV pilin multimeric outer membrane Mouse 2D-Western Blot Outer membrane Huntley et al. (2007), Twine et al. (2010)
FTT_1163 Hypothetical membrane protein Human, presumed type B; mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007), Sundaresh et al. (2007)
FTT_1201c Oxidoreductase Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2002, 2005)
FTT_1236 Hypothetical protein Mouse Proteome microarray Unknown Eyles et al. (2007)
FTT_1239 Hypothetical membrane protein Human, presumed type B Proteome microarray Cyt mem Sundaresh et al. (2007)
FTT_1269c Chaperone protein (heat shock protein family 70 protein) dnaK Human type A; human, presumed type B; mouse 2D-Western blot/proteome microarray Periplasmic space Havlasova et al. (2002, 2005), Twine et al. (2006a,b, 2010), Eyles et al. (2007), Janovska et al. (2007a,b), Sundaresh et al. (2007)
FTT_1270c Chaperone protein grpE Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_1271 Membrane-bound lytic murein transglycosylase A mltA Mouse Proteome microarray Unknown Eyles et al. (2007)
FTT_1281c Sigma 54 modulation protein yhbH Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_1303c Hypothetical protein Mouse; human, presumed type B 2D-Western blot/proteome microarray Unknown Sundaresh et al. (2007), Twine et al. (2010)
FTT_1313c Transcriptional elongation factor greA Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_1314c Type IV pili fiber building block protein Human, presumed type B; mouse Proteome microarray Unknown Eyles et al. (2007), Sundaresh et al. (2007)
FTT_1317c Inosine-5′-monophosphate dehydrogenase Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_1333 Hypothetical protein Human, presumed type B Proteome microarray Unknown Sundaresh et al. (2007)
FTT_1345 Hypothetical protein pdpB Mouse 2D-Western blot Outer Membrane Havlasova et al. (2002)
FTT_1357/1712 Intracellular growth locus, subunit C iglC Human, presumed type B; mouse 2D-Western blot Unknown Havlasova et al. (2002, 2005), Twine et al. (2006a)
FTT_1358/1713 Intracellular growth locus, subunit B iglB Human, type A; mouse 2D-Western blot/proteome microarray Unknown Eyles et al. (2007), Twine et al. (2010)
FTT_1368c Glyceraldehyde-3-phosphate dehydrogenase gapA Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_1369 Transketolase tktA Mouse 2D-Western blot Unknown
FTT_1373 3-Oxoacyl-[acyl-carrier-protein] synthase III Mouse 2D-Western blot Unknown Twine et al. (2010)
FTT_1374 Malonyl CoA acyl carrier protein fabD Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005), Twine et al. (2010)
FTT_1376 Acyl carrier protein acpP Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_1389 3-Methyl-2-oxobutanoate hydroxymethyl-transferase Mouse 2D-Western blot Unknown Twine et al. (2010)
FTT_1390 Pantoate-beta-alanine ligase panC Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_1402c Hypothetical protein Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_1406c Hypothetical protein Mouse Proteome microarray Unknown Eyles et al. (2007)
FTT_1416c Hypothetical lipoprotein Mouse Proteome microarray Unknown Eyles et al. (2007)
FTT_1441 Hypothetical protein Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005), Janovska et al. (2007a), Twine et al. (2010)
FTT_1444 Exopolyphosphatase ppx Mouse Proteome microarray Cytoplasmic membrane Eyles et al. (2007)
FTT_1460 UDP-glucose/GDP mannose dehydrogenase wbtE Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_1483c Dihydrolipoamide dehydrogenase Human, presumed type B 2D-Western blot Cytoplasmic Janovska et al. (2007a)
FTT_1484c Pyruvate dehydrogenase, E2 component aceF Human, type A; human, presumed type B; mouse 2D-Western blot/proteome microarray Cytoplasmic membrane Eyles et al. (2007), Janovska et al. (2007a,b), Sundaresh et al. (2007), Twine et al. (2010)
FTT_1485c Pyruvate dehydrogenase, subunit E1 Human, type A 2D-Western blot Cytoplasmic Janovska et al. (2007b)
FTT_1498 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha Human, presumed type B; mouse 2D-Western blot Unknown Janovska et al. (2007a), Havlasova et al. (2005)
FTT_1510 Aromatic amino acid transporter of the HAAAP family Human, presumed type B Proteome microarray Cytoplasmic membrane Sundaresh et al. (2007)
FTT_1526 Isocitrate dehydrogenase idh Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2005), Eyles et al. (2007), Janovska et al. (2007a)
FTT_1530 Fusion product of 3-hydroxyacyl-CoA dehydrogenase and acyl-CoA-binding protein fadB/acbP Mouse 2D-Western blot/proteome microarray Cytoplasmic Eyles et al. (2007), Twine et al. (2010)
FTT_1531 3-Ketoacyl-CoA thiolase Human, presumed type B 2D-Western blot Cytoplasmic Janovska et al. (2007a)
FTT_1533 Part of pseudogene of a sugar transport protein Mouse Proteome microarray Eyles et al. (2007)
FTT_1539c Hypothetical protein Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2005), Twine et al. (2006b), Eyles et al. (2007), Janovska et al. (2007a)
FTT_1540c Hypothetical protein Human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Eyles et al. (2007), Sundaresh et al. (2007), Twine et al. (2010)
FTT_1557c Two component response regulator Mouse 2D-Western blot Cytoplasmic Havlasova et al. (2005)
FTT_1572c Outer membrane protein OmpH oomph Mouse 2D-Western blot/proteome microarray Unknown Havlasova et al. (2005), Eyles et al. (2007)
FTT_1591 Lipoprotein Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_1569c UDP-N-acetylglucosamine acyltransferase lpxA Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_1610 Pseudogene Human, presumed type B Proteome microarray Unknown Sundaresh et al. (2007)
FTT_1616 Cysteinyl tRNA synthetase cysS Mouse 2D-Western blot Unknown Twine et al. (2006b)
FTT_1676 Hypothetical membrane protein Human, type A; human, presumed type B; mouse 2D-Western blot/proteome microarray Unknown Eyles et al. (2007), Janovska et al. (2007a,b)
FTT_1695 Chaperone protein, groES groES Human, presumed type B; mouse 2D-Western blot Cytoplasmic Havlasova et al. (2002, 2005)
FTT_1696 Chaperone protein, groEL groEL Human, presumed type B; mouse 2D-Western blot/proteome microarray Cytoplasmic Havlasova et al. (2002, 2005), Twine et al. (2006a,b, 2010), Eyles et al. (2007), Huntley et al. (2007), Janovska et al. (2007a), Sundaresh et al. (2007)
FTT_1702 Hypothetical protein Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_1712c Intracellular growth locus, subunit C iglC Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_1713c Intracellular growth locus, subunit B iglB Human, type A 2D-Western blot Unknown Janovska et al. (2007b)
FTT_1714c Intracellular growth locus, subunit A iglA Human, presumed type B 2D-Western blot Cytoplasmic Janovska et al. (2007a)
FTT_1724 Outer membrane protein tolC precursor tolC Human, presumed type B; mouse Proteome microarray Outer Membrane Eyles et al. (2007), Sundaresh et al. (2007)
FTT_1747 Outer membrane protein Mouse 2D-Western blot/proteome microarray Unknown Twine et al. (2006b), Eyles et al. (2007)
FTT_1749 Preprotein translocase, subunit B, chaperonin protein Human, presumed type B 2D-Western blot Unknown Janovska et al. (2007a)
FTT_1752 Single stranded binding protein ssb Mouse 2D-Western blot Unknown Havlasova et al. (2005)
FTT_1764c Ferredoxin Mouse 2D-Western blot Cytoplasmic Twine et al. (2006b)
FTT_1769 ClpB clpB Human, presumed type B; mouse 2D-Western blot Inner membrane Havlasova et al. (2005), Janovska et al. (2007a), Twine et al. (2010)
FTT_1775c Voltage-gated ClC-type chloride channel clcA clcA Human, presumed type B Proteome microarray Cytoplasmic membrane Sundaresh et al. (2007)
FTT_1778c Hypothetical membrane protein Mouse 2D-Western blot/proteome microarray Unknown Twine et al. (2006b, 2010), Eyles et al. (2007)

1Whether protein was reactive with sera from human infected with type A or type B strains of F. tularensis, or vaccinated mice.

2Method by which protein immunoreactivity was detected.

3Predicted protein subcellular location, using PSORT1b algorithm (Nakai and Horton, 1999; Gardy et al., 2003; Rey et al., 2005).