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. 2010 Apr 1;19(12):1949–1957. doi: 10.1089/scd.2009.0505

FIG. 5.

FIG. 5.

The modified reverse transfection (M-RT) protocol doubles the establishment of clonal human embryonic stem cell (hESC) lines. hESCs were transfected using either the forward transfection (FT) protocol or the M-RT 90 protocol with a plasmid that contains a dual prokaryotic/eukaryotic kanamycin/neomycin selectable marker cassette (pEGFP-N1). Seventy-two hours posttransfection, treatment with G418 was initiated to select G418-resistant hESC foci. (a) Photographs depict stably transfected H9-EGFP-N1 colonies stained with crystal violet. The transfection protocol used in each group is indicated above each image. (b) The graph illustrates the fold increase in number of G418-resistant foci obtained using M-RT compared to FT group and control group. Nontransfected cells were used as a control group.

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