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. 2000 Oct;10(10):1605–1616. doi: 10.1101/gr.151700

Figure 3.

Figure 3

Cytogenetic fine structure FISH analysis of ExtP1-7. Direct-labeled FISH probes were used to visualize the extended P element, ExtP1-7, on the X chromosome. Three-color staining allowed simultaneous detection of chromatin (DAPI images, panels A,E), the boundaries of the extended P elements (pP{LacW} FISH signals—FITC channel, panels B,F), and the interior portion of the extended P (P1-DS04361 FISH signals—rhodamine channel, panels C,G). A close-up view of the pseudocolored overlay image (panel I) reveals the cytogenetic fine structure of the extended P element. The FISH images show good correspondence between the FISH staining patterns (I) and the structure of the extended P element (H; see Fig. 1).