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. 2011 Feb 4;13(1):R17. doi: 10.1186/bcr2826

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Copyright ©2011 Ladjemi et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anti-HER2 antibodies are present in sera from BALB/c mice immunized with anti-Id scFv40 and scFv69. (A) ELISA of pooled sera from mice (n = 20/group) immunized with scFv40, scFv69 or HER2-Fc. Values are presented as mean ± SD of the Absorbance at 490 nm from three independent experiments. Flow cytometry analysis in (B) HER2-overexpressing SK-OV-3 and (C) HER2-negative CHO cells of the HER2-specific response of pooled sera from mice immunized with PBS, anti-Id scFv40, scFv69 or with HER2-Fc. Sera were recovered at Day 42 post-vaccination. Antibody binding was detected using a FITC-labeled goat anti-mouse antibody. FITC-labeled secondary antibody alone was used as a negative control to determine the level of background fluorescence.