Table 1.

Cytokine secretion in antigen-stimpulated splenocytes from MMTV.f.huHER2(Fo5) transgenic mice immunized with anti-idiotype scFv 69 vaccine

Cytokine	Conc	HER2-transgenic mouse immunized with
Stimulating antigen	(μg/ml)
		PBS	scFv69
Interleukin-2
Con A	7.5	356.9 ± 5.7	422.8 ± 26.5
Control scFv 13R4	2.0	<4	<4
ScFv69	2.0	<4	10.6 ± 0.8
Control Mfe23-Fc	2.0	<4	<4
HER2-Fc	2.0	<4	<4
Interferon-gamma
Con A	7.5	21.4 ± 0.9	24.2 ± 0.8
Control scFv 13R4	2.0	<1	7.0 ± 3.6
ScFv69	2.0	<1	17.0 ± 3.7
Control Mfe23-Fc	2.0	<1	<1
HER2-Fc	2.0	<1	<1

*ng/ml.

Cytokine (Il-2 and IFN-gamma) secretion in splenocytes from MMTV.f.huHER2(Fo5) transgenic mice immunized with PBS or anti-Id scFv69. Splenocytes were isolated on Day 42 post-immunization. Supernatants from antigen-stimulated cells were recovered 24 h (for IL-2) and 72 h (for IFN-gamma) after in vitro stimulation. IL-2 and IFN-gamma detection (ng/ml) were performed using an ELISA assay, with a standard curve obtained with recombinant IL-2 (detection limit 4 ng/ml) or recombinant IFN-gamma (detection limit 1 ng/ml). Results are the mean ± SD of triplicate wells and are representative of two independent experiments. Mfe23-Fc and scFv13R4 are two irrelevant antigens. Con A, Concanavalin A.