Figure 14.22.2.

Thioglycollate-elicited macrophages treated with varying concentrations of cytokine cocktail (IL-4/IL-13/GM-CSF) for 24 hr and pulsed with OVA peptide were co-cultured with CFSE-labeled OT-II T cells for 72 hr. Observe the potent inhibition of T cell proliferation even at a dose of 100 pg/ml of the cytokine cocktail. Note that the gate is set on CD4⁺ cells during analysis and that dilution of CFSE intensity denotes proliferation.