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. Author manuscript; available in PMC: 2012 Jun 6.
Published in final edited form as: Vaccine. 2011 Apr 16;29(25):4210–4217. doi: 10.1016/j.vaccine.2011.03.102

Fig. 4.

Fig. 4

Binding of the rabbit serum against O35Elgt5 dLOS-TT to the surface of M. catarrhalis as visualized by immuno-electron microscopy. Rabbits were injected subcutaneously (1 dose every 4 weeks, 3 doses total) with O35Elgt5 dLOS-TT (50 μg of carbohydrate each rabbit) and Sigma Adjuvant System. The rabbits were bled before the immunization and 2 weeks after each injection. Cells of strains O35E (type A) (A), 25238 (type A) (B), 26397 (type B) (C), and 26404 (type C) (D) were incubated with a rabbit serum against O35Elgt5 dLOS-TT plus the adjuvant after three injections (1:200 dilution). Cells of strain 25238 (E) were also incubated with a corresponding rabbit preimmune serum (1:200 dilution) as a control. Data are representative of three independent experiments. Gold particles are 5 nm in diameter. Scale bars, 100 nm.