FIGURE 2.

Cytotoxic activity of IEL against E. cuniculi-infected target is perforin-dependent. IEL from WT mice were purified at day 7 p.i. (n = 6 mice/group). A, IELs were labeled for cytotoxic markers (perforin, CD95L, and granzyme B). Cells were isolated at day 7 p.i., and expression of perforin, granzyme B, and CD95L were analyzed by intracellular staining. B, Before incubation with ⁵¹Cr-labeled uninfected and infected macrophages (E:T ratio of 20:1), IEL were treated with either CMA or anti-FasL Abs. After 4 h of incubation, the cytolytic activity was determined by radioisotope release into the culture supernatant. The experiment was performed twice with similar results. Data are representative of one set of experiments.