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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: J Neuroimmunol. 2006 Apr 21;175(1-2):19–30. doi: 10.1016/j.jneuroim.2006.02.015

Table 5. Validation of microarray data using RT-PCR.

Gene Fold changea Change in gene vs. change in referenceb
IIGP 296 368
TGTP 203 252
IRG-47 69 86
IGTP 43 54
GTPI 28 35
LRG-47 15 18

Fold change was calculated using the difference between Ct values between IFN-γ treated vs. untreated astrocytes for each of the different p47 GTPases and also using actin as a reference gene and change in gene vs. change in reference calculated using the Pfaffl method (Pfaffl, 2001).

a

Fold change=Difference in Ct between IFN-γ treated vs. untreated astrocytes.

b

Change in gene as determined using actin as a reference using the Pfaffl method.