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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: Trans R Soc Trop Med Hyg. 2007 Apr 6;101(6):547–554. doi: 10.1016/j.trstmh.2007.02.005

Table 2.

Comparison of two DNA pretreatment methods for PCR detection of microsporidia and the trichrome stain in a randomly selected set of stool samples

Samplea Origin Conventional
PCR-RFLP		 Cycle threshold values
for real-time PCR		 Modified trichrome
(number of spores/g
of stool)
Beads disruption Alkaline treatment
W192 Hospital E. bieneusi 28.4 29.1 240 000
P85 School Negative NA NA 0
W172 Hospital Negative NA NA 0
307 Hospital E. bieneusi 34.7 34.2 1500
B110 Hospital E. bieneusi 37.2 37.9 600
P125 School E. bieneusi 34.2 34.8 1800
P261 School Negative NA NA 0
08 Hospital E. bieneusi 36.4 36.2 0
W181 Hospital E. bieneusi 33 32.1 3300
B16 Hospital E. bieneusi 34.88 35.68 1200
B200 Hospital E. bieneusi 33.6 33.8 6000
B175 Hospital E. bieneusi 34.4 34.6 5800
158 Hospital E. bieneusi 34.4 35 1000
C40 Hospital Negative NA NA 0
P82 School Negative NA NA 0
B177 Hospital E. bieneusi 31.8 32.5 90 000
P22 Hospital Negative NA NA 0
W169 Hospital E. bieneusi 34.6 35.22 0
13 Hospital E. bieneusi 35 36 0
P89 School Negative NA NA 0
E. bieneusi DNA (3 ng) Positive 23.4 – NA
E. bieneusi DNA (0.3 ng) Positive 30.2 – NA

NA: not applicable.

a

All the positive samples and a few negative by both methods (PCRs and microscopy) are included.