Fig. (2).

Competition of hLF binding to epithelial cells by the LF-33 peptide. HEC-1 and HT-29 cells were incubated with hLF (20 µg or 256 nM) or LF-33 peptide (20 µg or 5 µM) for 30 min at 4°C, washed to eliminate the hLF and peptide excess before to be incubated with fluorescent-hLF (20 µg) for 1 h at 4°C and analysed by flow cytometry. Histograms depict the mean ± SD of binding (%) and mean fluorescences calculated on M2 gate obtained from 3 independent experiments.