Polymerase chain reaction using primers M2::M4 of microsporidian DNA demonstrating the presence of microsporidian MetAP2 amplicons. The ~500 bp amplicons are consistent with the predicted size of a MetAP2 amplicon based on the location of these primers in other eukaryotic MetAP2 sequences. A 100 bp ladder is used as the molecular weight standard. C – control with no DNA. Ba – Brachiola algerae, Ga – Glugea americanus, Ei – Encephalitozoon intestinalis, Ec – Encephalitozoon cuniculi, Eh – Encephalitozoon hellem,Eb – Enterocytozoon bieneusi.