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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: Folia Parasitol (Praha). 2005 May;52(1-2):182–192. doi: 10.14411/fp.2005.023

Fig. 4.

Fig. 4

Reactivity of native and recombinant microsporidian MetAP2 with antibodies to human and Encephalitozoon MetAP2. A. Immunoblot using anti-cHuMetAP2. This antibody reacted with a ~40-kDa protein in Encephalitozoon cuniculi spore lysates (lane 1) and to rEcMetAP2 expressed in Sf9 cells (lane 2). No reactivity was seen in Sf9 cells without the PVL1392-EcMetAP2 construct (data not shown). B. Immunoblot using anti-rEcMetAP2. This anti-rEcMetAP2 reacted with a ~40-kDa protein in E. cuniculi spore lysates (lane 1) and to rEcMetAP2 expressed in Sf9 cells (lane 2). No reactivity was seen in Sf9 cells without PVL1392-EcMetAP2 (data not shown). (Reprinted with permission from Weiss et al. 2003.)