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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: Folia Parasitol (Praha). 2005 May;52(1-2):182–192. doi: 10.14411/fp.2005.023

Fig. 5.

Fig. 5

Purification of recombinant Encephalitozoon cuniculi methionine aminopeptidase type 2 (rEcMetAP2). An AKTA prime FPLC employing a Sepharose 6 column was used to isolate rEcMetAP2 produced from baculovirus (PVL1392-EcMetAP2) infected Sf9 cells. The arrow points to peak from the fractions corresponding to EcMetAP2. Insert A: Coomassie blue stain of SDS-PAGE demonstrating a single protein of expected size in this peak. Insert B: Immunoblot using anti-EcMetAP2 demonstrating reactivity of the purified protein with antibody to EcMetAP2.