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. 2011 Mar 30;117(20):5350–5361. doi: 10.1182/blood-2011-01-333476

Figure 6.

Figure 6

Kit down-regulation in SLAM marker-labeled HSCs. (A) Representative FACS plots for lin−Sca1+CD48−CD150+ staining 4 weeks post final injection indicates severe reduction of Kit expression in SLAM marker-enriched HSCs from fl/fl:cre+ BM. (B) Median fluorescence intensity (MFI) for Kit staining of lin−Sca1+CD48−CD150+ cells (n = 4). (C) FACS histogram plots illustrating Kit staining of lin−Sca1+CD48−CD150+ cells at different time points after first poly-I:C injection, indicating immediate Kit down-regulation after acute Shp2 deletion. (D) Immunoblotting of BM cells after first poly-I:C injection showed gradually increased deletion from day 7 to 16. Tubulin was used as loading control. ***P < .001, **P < .01,*P < .05, error bars are SD; see also supplemental Figure 4.