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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: J Phys Chem C Nanomater Interfaces. 2011 Feb 10;115(5):1410–1414. doi: 10.1021/jp106912p

Figure 2.

Figure 2

Experiment schematic and LSPR spectra. (A) Biotin is covalently linked to the nanoparticle surface using EDC coupling agent, and antibiotin labeled gold nanoparticles are subsequently exposed to the surface. LSPR spectra are collected before and after each step. (B) LSPR spectra before (solid black) and after (dashed blue) binding of native antibiotin, showing a Δλmax of 11 nm. (C) LSPR spectra before (solid black) and after (dashed red) binding of antibiotin labeled nanoparticles, showing a Δλmax of 42.7 nm.