Figure 3.
Classification and averaging of negatively-stained particle images and fit of octameric C3PO into 3D EM map. (a) Montage of nine panels for comparison between reprojection of the reconstruction (image I) with the corresponding class average (image II) showing a representative particle in that class (image III). (b) Docking of the octameric full-length C3PO (Translin:Trax ratio of 6:2) model in the 3D EM map. Trax (pink) and Translin (cyan) molecules are shown in ribbon representation. Both 6:2 and 5:3 Translin:Trax models of C3PO could be fitted satisfactorily within the EM envelope. Top and side views related by a 90° rotation are shown in left and right panels, respectively.