TABLE 4.
Amoeba cultures and corresponding Legionella 16S sequence identification and amoeba 18S sequence identification.
| Amoeba culture number | Culture Name | Putative Legionella-like 16S sequence | Bacterial Blast Result | Putative amoeba 18S sequence | Amoeba Blast resul |
|---|---|---|---|---|---|
| 1 | 04/05 CO 996.3 | L. lyticum | 100% cov/ 100% ID | Cercozoan | 100% cov/ 97% ID |
| 2 | 04/05 CO MNN.2 | L. lyticum | 98% cov/ 99% ID | ||
| 3 | 06/05 CO 997.1 | Unc. Legionella Tsw8-2 | 100% cov/ 97% ID | ||
| 5 | 06/05 CO 997.3 | Unc. Legionella clone 20 | 100% cov/ 96% ID | ||
| 6 | 06/05 CO 994.1 | Unc. Legionella clone 20 | 98% cov/ 98% ID | ||
| 7 | 06/05 CO FNN.1 | Unc. Legionella Tsw8-2 | 100% cov/ 97% ID | Platyamoeba sp. | 99% cov/ 93% ID |
| 8 | 06/05 CO FNN.2 | L. pneumophila | 100% cov/ 96% ID | ||
| 9 | 06/05 BR MNN.1 | L. worsliensis | 100% cov/ 97% ID | Reticulamoeba sp. | 92% cov/ 90% ID |
| 10 | 06/05 BR 996.1 | Unc. Legionella Sb1-1 | 100% cov/ 96% ID | Flabellula sp | 91% cov/ 99% ID |
| 11 | 06/05 BR 996.2 | Unc. Legionella Tsw8-2 | 100% cov/ 97% ID | Paratetramitus sp. | 100% cov/ 99% ID |
| 12 | 06/05 BR FNN.2 | Unc. LegionellaSb1-1 | 100% cov/ 96% ID | ||
| 13 | 06/05 BP MNN.2 | Unc. Legionella; Sb1-1 | 100% cov/ 96% ID | ||
| 14 | 06/05 BP MNN.3 | Unc. Legionella S4-7 | 100% cov/ 98% ID | ||
| 15 | 06/05 BP 997.1 | Singhamoeba sp. | 100% cov/ 98% ID | ||
| 16 | 06/05 BP FNN.2 | L. londiniensis | 100% cov/ 96% ID | ||
| 17 | 06/05 DB MNN.1 | Unc. Legionella clone 27 | 100% cov/ 95% ID | Cercozoan/Protaspis | 100% cov/ 90% ID |
| 18 | 06/05 DB 997.1 | L. pneumophila | 100% cov/ 96% ID | ||
| 19 | 06/05 DB 997.3 | Unc. Legionella Tsw8-2 | 100% cov/ 97% ID | ||
| 20 | 06/05 DB 994.2 | Unc. Legionella Tsw8-2 | 100% cov/ 96% ID | ||
| 21 | 06/05 DB 996.2 | Unc. Legionella Tsw8-2 | 100% cov/ 97% ID | Acanthamoeba polyphaga | 99% cov/ 99% ID |
| 22 | 07/05 BR 994.1 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | Didymium sp. | 99% cov/ 87% ID |
| 23 | 07/05 BR MNN.1 | Unc. LegionellaTsw5-3 | 99% cov/ 98% ID | ||
| 24 | 07/05 BR MNN.2 | Unc. Legionella clone 45 | |||
| 25 | 07/05 BR MNN.3 | Unc. Legionella clone 46 | 100% cov/ 99% ID | Cercozoan | 92% cov/ 99% ID |
| 26 | 07/05 DB 997.1 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | Acanthamoeba sp. | 99% cov/ 98% ID |
| 28 | 07/05 DB 994.1 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | ||
| 29 | 07/05 DB 994.3 | Unc. LegionellaTsw5-3 | 100% cov/ 98% ID | ||
| 31 | 07/05 BP MNN.3 | Unc. Legionella clone LS-16 | 100% cov/ 96% ID | ||
| 32 | 07/05 BP 994.1 | Unc. Legionella clone LS-16 | 98% cov/ 99% ID | ||
| 33 | 07/05 BP 997.2 | Unc. Legionella clone SAM09-34 | 99% cov/ 97% ID | ||
| 34 | 07/05 BP 997.3 | Unc. Legionella clone LS-16 | 100% cov/ 97% ID | ||
| 35 | 08/05 BP 994.2 | Unc. Legionella Sb1-2 | 99% cov/ 98% ID | ||
| 36 | 08/05 BP 994.3 | Unc. Legionella Tag4-3 | 100% cov/ 98% ID | Putative cercozoan | 87% cov/ 86% ID |
| 37 | 08/05 BP 994.4 | Unc. Legionella Tsw5-3 | 100% cov/ 97% ID | ||
| 38 | 08/05 BP MNN.1 | Unc. Legionella Sb1-2 | 99% cov/ 97% ID | Neoparamoeba sp. | 100% cov/ 97% ID |
| 39 | 08/05 BR MNN.1 | Unc. Legionella Tang1-1 | 100% cov/ 98% ID | ||
| 40 | 08/05 BR MNN.2 | Unc. Legionella Tsw5-3 | 99% cov/ 95% ID | ||
| 42 | 08/05 BR MNN.4 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | ||
| 43 | 08/05 BR 996.1 | Rheinheimera aquimaris | 98% cov/ 99% ID | Acanthamoeba sp. | 99% cov/ 99% ID |
| 45 | 08/05 BR 996.3 | Rheinheimera aquimaris | 98% cov/ 99% ID | Acanthamoeba sp. | 100% cov/ 99% ID |
| 46 | 08/05 CO 994.1 | L. anisa | 99% cov/ 99% ID | ||
| 47 | 08/05 CO 994.2 | Unc. Legionella Tsw5-3 | 100% cov/ 98% ID | ||
| 48 | 08/05 CO 994.3 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | Cercozoan | 96% cov/ 98% ID |
| 49 | 08/05 CO 994.4 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | ||
| 50 | 08/05 CO 994.5 | Unc. Legionella Clone 46 | 100% cov/ 98% ID | ||
| 52 | 08/05 CO MNN.2 | Unc. Legionella S5-1 | 98% cov/ 96% ID | ||
| 54 | 08/05 CO MNN.5 | L. anisa | 100% cov/ 99% ID | ||
| 55 | 08/05 DB 996.1 | Rheinheimera aquimaris | 99% cov/ 99% ID | Acanthamoeba lugdunensis | 100% cov/ 99% ID |
| 56 | 08/05 DB 996.2 | Rheinheimera aquimaris | 98% cov/ 99% ID | Acanthamoeba sp. | 99% cov/ 99% ID |
| 57 | 08/05 DB 996.3 | Unc. Legionella Tsw5-3 | 99% cov/ 97% ID | Acanthamoeba lugdunensis | 100% cov/ 99% ID |
| 58 | 08/05 DB FNN.1 | Unc. Legionella S6-1 | 99% cov/ 96% ID | ||
| 59 | 08/05 DB 994.1 | Rheinheimera aquimaris | 99% cov/ 99% ID | ||
| 60 | 08/05 DB 994.2 | Rheinheimera aquimaris | 98% cov/ 98% ID | ||
| 61 | 08/05 DB 994.3 | Unc. Legionella Sb1-2 | 99% cov/ 98% ID | Vannella sp. | 99% cov/ 99% ID |
| 62 | 08/05 DB 994.5 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | Vannella sp. | 99% cov/ 99% ID |
| 63 | 08/05 DB 997.2 | L. anisa | 100% cov/ 99% ID | Hartmannella sp. | 99% cov/ 99% ID |
| 64 | 08/05 DB 997.3 | Unc. Legionella Tang2-1 | 98% cov/ 98% ID | Naegleria sp. | 99% cov/ 99% ID |
| 65 | 08/05 DB 997.4 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | ||
| 66 | 08/05 DB 997.5 | Unc. Legionella Sb1-2 | 99% cov/ 98% ID | ||
| 67 | 08/05 DB MNN.1 | Unc. Legionella Clone 20 | 100% cov/ 97% ID | ||
| 68 | 08/05 DB MNN.2 | Unc. Legionella Tang1-9 | 100% cov/ 97% ID | ||
| 69 | 08/05 DB MNN.3 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | ||
| 70 | 08/05 DB MNN.4 | Unc. Legionella Sb1-2 | 99% cov/ 98% ID | ||
| 71 | 08/05 DB MNN.5 | Unc. Legionella Tsw5-3 | 99% cov/ 98% ID | ||
| 72 | 08/05 DB 994.4 | Unc. Legionella Sb1-2 | 99% cov/ 98% ID | Vannella sp. | 99% cov/ 99% ID |
| 73 | 06/06 BR 996.1 | L. moravica | 100% cov/ 99% ID |
Paraflabellula sp. (73) Filamoeba sp. (73b) |
100% cov/ 98% ID 99% cov/99% ID |
| 74 | 06/06 BR 996.2 | L. moravica | 100% cov/ 99% ID | ||
| 75 | 06/06 CO 994.5 | L. impletisoli | 100% cov/ 94 % ID | ||
| 76 | 06/06 CO 994.6 | L. impletisoli | 100% cov/ 94 % ID | ||
| 77 | 06/06 CO 994.7 | Unc. Bacteria UC04 | 99% cov/ 97% ID | ||
| 78 | 06/06 DB 996.2 | L. moravica | 100% cov/ 99% ID | ||
| 79 | 06/06 DB 996.3 | L. longbeachae | 100% cov/ 92% ID | ||
| 80 | 06/06 DB 997.2 | Unc. Legionella Tang7-1 | 99% cov/ 97% ID | ||
| 81 | 06/06 BP 994.7 | Unc. Beta-proteobacteria | 99% cov/ 99% ID | ||
| 82 | 06/06 BP 994.8 | Unc. Legionella S6-1 | 99% cov/ 98% ID | ||
| 84 | 06/06 BP 997.2 | L. drancourtii LLAP 12 | 100% cov/ 99% ID | Hyperamoeba sp. | 97% cov/ 91% ID |
| 85 | 06/06 BP 997.3 | L. drancourtii LLAP 12 | 100% cov/ 99% ID | ||
| 87 | 06/06 CO 996.2 | L. drancourtii LLAP 12 | 100% cov/ 99% ID | Filamoeba sp. | 99% cov/ 98% ID |
| 88 | 06/06 CO FNN.1 | Rheinheimera sp. | 99% cov/ 100% ID | Filamoeba sp. | 99% cov/ 98% ID |
| 89 | 06/06 CO FNN.2 | Unc. Legionella Tsw8-5 | 99% cov/ 99% ID | ||
| 90 | 06/06 CO MNN.2 | Unc. Legionella Tag6-1 | 98% cov/ 97% ID | ||
| 92 | 06/06 DB FNN.2 | L. drancourtii LLAP 12 | 100% cov/ 99% ID | ||
| 93 | 06/06 DB MNN.3 | Unc. Legionella SAM09-34 | 99% cov/ 96% ID | ||
| 94 | 06/06 BP FNN.1 | Unc. Legionella Sb1-7 | 100% cov/ 98% ID | ||
| 96 | 02/06 BR 997 | L. quateirensis | 100% cov/ 98% ID | Reticulamoeba sp. | 96% cov/ 96% ID |
| 98 | 02/06 BR MNN.5 | Unc. Bacteria 103mbsf_2_8A | 94% cov/ 99% ID | Reticulamoeba sp. | 98% cov/ 96% ID |
| 99 | 02/06 BR MNN.7 | Unc. Bacteria 103mbsf_2_8A | 98% cov/ 99% ID |
Reticulamoeba sp. (99) Nolandella sp. (99a) |
98% cov/ 95% ID 97% cov/ 99% ID |
| 101 | 02/06 CO FNN.9 | L. worsliensis | 100% cov/ 99% ID | Filamoeba sp. | 99% cov/ 99% ID |
| 102 | 02/06 CO FNN.2 | LLAP 10 | 100% cov/ 100% ID | ||
| 103 | 02/06 CO FNN.4 | L. quateirensis | 99% cov/ 98% ID | Naegleria sp. | 100% cov/ 99% ID |
Culture Name indicates collection date (month/year), location (CO - Common Fence Point, DB - Dighton Bridge, BR – Braga Bridge, BP – Brayton Point), and culture medium with isolate number (e.g. 996.3).