Figure 5.

Lack of cross talk between D₁-like receptors and Gal₂ receptors on ERK1/2 phosphorylation in transfected cells. a, b, Cells were cotransfected with the cDNA corresponding to D₅ (1.5 μg) and Gal₂ (2 μg) receptors (a) or to D₁ (1 μg) and Gal₂ (2 μg) receptors (b). Cells were treated for 5 min with the indicated concentrations of galanin in the absence (circles) or in the presence (triangles) of 50 nm (a) or 70 nm (b) of SKF 81297. The immunoreactive bands from four independent experiments were quantified and the values represent the mean ± SEM of phosphorylation (arbitrary units) minus the basal levels found in untreated cells.