Fig. 4.

Co-localization of Snail2 and Runx2 in a high-grade tibial osteosarcomas. H&E stain (A, E, I), immunolocalisation of Snail2 (B, F, J), Runx 2 (C, G, K) and co-localization (D, H, L). High-grade tumours are shown (A–H) and low-grade tumour (I–L). Boxed area shows regions used for Snail2 and Runx2 co-localization. In high-grade tumours, Snail2 and Runx2 co-localise (arrowheads in D and H). Arrowheads indicate the same cells expressing both proteins. Inserts in B–D are ×100 magnification. Scale bar = 10 μm