Figure 11.

Double-labeling immunofluorescence analysis of Drp1 and COX1 in primary neurons from AβPP transgenic mice and wild-type mice. Drp1 and COX1 colocalize in cell body, neruites and growth cones. Hippocampal neurons from wild-type or AβPP mice were immunostained for Drp1, mitochondrial marker COX1 and DAPI. Hippocampal neurons from wild-type mice (upper panels) (Drp1, A; COX1, B; DAPI, C; and merged, D) show Drp1 immunoreactivity in growth cones and neurites, whereas neurons from AβPP mice (lower panels) (Drp1, E; COX1, F; DAPI, G; and merged, H) show Drp1 immunoreactivity mainly in the cell body and axons next to the cell body. Areas of active growth were not commonly observed in AβPP cultures. In both wild-type and AβPP cultures Drp1 colocalized with mitochondrial-encoded protein, COX1.