Figure 3.

Chromosome aberrations in HeLa cells after depletion of SNM1B, FANCD2 or FANCI. (Upper panel) HeLa cells were transfected with the indicated siRNAs and treated with MMC (20 ng/ml) for 24 h. Metaphases were prepared and scored for the presence of gaps/breaks and radial chromosomes. Graph indicates the percentage of metaphases containing at least one gap/break or radial. Error bars are standard error. (Lower panel) The total number of metaphases scored for each sample, and the number of metaphases containing one or more aberrations, gaps/breaks or radials is shown. P-values for pairwise comparisons (two-tailed F-test) are shown in Supplemental Material, Table S1.