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. 2011 Apr 8;20(13):2549–2559. doi: 10.1093/hmg/ddr153

Figure 4.

Figure 4.

MMC-induced BRCA1 and RAD51 foci formation are impaired in SNM1B-depleted cells. (A) BRCA1 foci formation in SNM1B-depleted cells. HeLa cells transfected with NS or siSNM1B-2 were treated with MMC (330 ng/ml) for 8 h at 48 h post-transfection. Cells were fixed with p-formaldehyde and stained with α-BRCA1 antibodies (green). Nuclei were stained with DAPI (blue). The percentage of cells containing >10 foci was determined. (B) RAD51 foci formation in SNM1B-depleted cells. siSNM1B-1- or NS-transfected cells were fixed with p-formaldehyde and stained with α-RAD51 antibodies (green). Nuclei were stained with DAPI (blue). The percentage of cells containing >10 foci was determined. At least 300 nuclei for each sample were scored. Representative images are shown. All images were required at ×100 magnification. Graphs represent average of three independent experiments with standard deviation. *P< 0.05; **P< 0.005.