Figure 5.

SNM1B promotes homology-mediated repair of DNA DSBs. (A) Schematic of DR–GFP construct containing a GFP cassette interrupted by an I-SceI recognition site. Upon I-SceI expression, a DSB is generated, and GFP expression is restored upon homologous recombinational repair using a downstream internal GFP fragment (iGFP). HeLa cells containing the chromosomally integrated DR–GFP reporter construct were transfected with the indicated siRNAs. At 24 h post-transfection, cells were infected with adenovirus expressing the I-SceI nuclease. Cells were collected after 48 h and the percentage of GFP-positive cells was determined by flow cytometry. (B) Relative HR frequencies. The relative HR frequencies of SNM1B, FANCD2, SNM1B/FANCD2 and RAD51-depleted cells were calculated based on the percentage of GFP-positive cells relative to that observed in control (NS) cells. Graph represents the average of three independent experiments. Bars are standard deviations. *P< 0.05; **P< 0.005, Student's two-tailed t-test.