Table 2.

Recovery rates of the analytes determined in spiked urine, plasma and whole blood samples. The mean of the measured concentration, the recovery rate and RSD of the analysis of 3 independent samples (inter-sample variation) is shown. Intra-sample variation is represented by the mean of the RSD of three injections of the same sample

Matrix (spiked content)	Measured concentration/nM	Recovery rate (%)	Intra-sample RSD (%)	Inter-sample RSD (%)
Urine (10 nM)	9.98	99.83	4.18	4.67
Urine (30 nM)	30.43	101.4	0.97	5.70
Urine (100 nM)	103.6	103.6	0.73	5.37
Plasma (10 nM)	10.57	105.7	3.80	8.71
Plasma (30 nM)	32.99	110.0	2.58	7.89
Plasma (100 nM)	102.3	102.3	2.00	4.93
Whole blood (10 nM)	9.95	99.53	3.19	1.98
Whole blood (30 nM)	30.35	101.2	2.56	6.64
Whole blood (100 nM)	100.3	100.3	1.63	3.84