. Author manuscript; available in PMC: 2011 Jun 7.

Published in final edited form as: Anal Methods. 2011 Jan 1;3(2):420–428. doi: 10.1039/C0AY00714E

Table 3.

Application of the method on rofecoxib (ROF) and inhibitors of soluble epoxide hydrolase (sEHi) a–g (Fig. 7). The retention times, transition used for detection by ESI(−)MS/MS, limit of detection and linear range are shown

Compound	Retention time/min^a	MS/MS transition m/z	Compound MS conditions		Limit of detection/nM	Linear range/nM
Compound	Retention time/min^a	MS/MS transition m/z	DP/V	CE/V	Limit of detection/nM	Linear range/nM
ROF	1.488	313/285	−65	−24	0.3	1–1000
sEHi a	1.595	380/176	−100	−24	0.01	0.03–300
sEHi b	1.743	394/176	−100	−22	n.d.^b (I.S)	n.d.^b (I.S)
sEHi c	1.360	344/176	−95	−20	0.1	0.3–300
sEHi d	1.300	308/126	−85	−20	0.01	0.03–300
sEHi e	1.735	437/260	−95	−22	0.1	0.3–1000
sEHi f	1.823	411/137	−115	−34	0.3	1–1000
sEHi g	1.955	339/214	−70	−34	1	3–1000

RSD < 0.5%.

n.d. = not determined (I.S. compound).