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. 2011 Mar 4;117(21):5733–5743. doi: 10.1182/blood-2009-09-242131

Figure 2.

Figure 2

Exogenous NmU peptide rescues impaired BFU-E and CFU-E production from primary human CD34+ cells in which endogenous NmU is silenced. Human primary CD34+ cells nucleofected with control, NmU, or NMUR1 siRNA were cultured in methylcellulose. (A) At 24 hours after nucleofection, NmU expression was determined by quantitative real-time PCR. The data represent the mean of a triplicate determination. The frequencies of (B) CFU-GM, (C) BFU-E, and (D) CFU-E derived from control and NmU siRNA-treated CD34+ cells are presented from duplicate determinations from 2 independent experiments. White bars represent control siRNA-treated cells; black bars, NmU siRNA-treated cells; dark gray bars, NmU siRNA-treated cells cultured with exogenous NmU peptide; and light gray bars, NmU siRNA-treated cells cultured with exogenous neurotensin. (E) NMUR1 expression in CD34+ cells 24 hours after nucleofection of NMUR1 siRNA was determined by quantitative real-time PCR. The frequencies of (F) BFU-E and (G) CFU-E produced from control and NMUR1 siRNA-treated cells are presented from duplicate determinations from 3 independent experiments. The white bars represent control siRNA-treated cells; black bars, NMUR1 siRNA-treated cells; dark gray bars, NMUR1-treated cells cultured with exogenous NmU peptide; and light gray bars, NMUR1-treated cells cultured with exogenous neurotensin.