Table 1.
PCR Primers and Reaction Conditions
| Symbol | Primer name | Product size (bp) | Primer sequence | Reaction conditionsa | Accession no. | |
|---|---|---|---|---|---|---|
| D10Jhu25 | 100K2.T7 | 104 | F-5′TACTTCAGGGTGTGTGGCAG | R-5′ACAGGGTGAGGAGGGACC | Prog 45 | AF167489 |
| D10Jhu26 | 100K2.SP6 | 480 | F-5′CCTAGGTGAAGCGTCCAGAG | R-5′GGGGACTTAAAAAGCTTGCC | Prog 30 | AF167488 |
| D10Jhu27 | 386A2.T7 | 223 | F-5′ACTCAACCTGGGCCTCAAC | R-5′TCACCTAGCGTGAGTGCAAG | Prog 30 | AF167518 |
| D10Jhu33 | 599G22.SP6 | 125 | F-5′TCAGCGGGAAATGACATACA | R-5′TGGGGATCTTCAGTGACACA | Prog 42 | AF167508 |
| D10Jhu34e | 300O21.T7 | 186 | F-5′TGAGTGCCTCAGAAGAGGGT | R-5′AGAACTCTGGTGCGGTCAGT | Prog 45 | AF167501 |
| D10Jhu35 | 247L16.T7 | 256 | F-5′CCAAGACTGTGTGCCAGAAC | R-5′GCAGTTAGAACTTCCGTCCG | Prog 42 | AF167498 |
| D10Jhu36 | 331J21.SP6 | 419 | F-5′GGGAGTTGGGGAGATACTGG | R-5′CATGCTTGCTTCTGCTTGAC | Prog 30 | AF164436 |
| D10Jhu37 | 235F4.SP6 | 146 | F-5′CGCTAAAGGTGGATGCTTTC | R-5′CTGTCCGGGAACACATGAC | Prog 10 at 57 | AF167496 |
| D10Jhu38 | 247L16.SP6 | 287 | F-5′TTCCTGTCCCATCTCCAGAC | R-5′TGATTCCAGTCAAGCCTTCC | Prog 42 | AF167497 |
| D10Jhu39 | 331J21.T7 | 314 | F-5′ACCCATATGCAGAGACCCAG | R-5′CCTCATCTGCCAACAATCAG | Prog 30 | AF167502 |
| D10Jhu40 | 628P5.SP6 | 260 | F-5′CCGCCAAGTGCTAGATTTTC | R-5′GTAGCTAAGCGGCTCCTGTG | Prog 1 at 60 | AF167509 |
| D10Jhu41 | 64013.SP6 | 391 | F-5′CAGCTAACTAAAGCCACGGG | R-5′TCACTGCTACCAATGCCAAG | Prog 30 | AF167528 |
| D10Jhu42 | 166J15.T7 | 238 | F-5′ACATTGCCTGGTCCTCTCTG | R-5′AGCCACATGGCTCCTATCAG | Prog 10 at 57 | AF167495 |
| D10Jhu43 | 578K13.T7 | 198 | F-5′TTTGCTGGGGGTACAAGTTC | R-5′CTAAACAGATGCCCCTGGAC | Prog 10 | AF167507 |
| D10Jhu44 | 64012.T7 | 252 | F-5′CTCGACCTTTTCTGTCCTCG | R-5′CTTTCCGTATACGCGATTCC | Prog 10 | AF167529 |
| D10Jhu45 | 628P5.T7 | 140 | F-5′CCAGGAGGAGGAAGCTGTAG | R-5′AATACCCCCTTCCACCACTC | Prog 10 at 60 | AF167510 |
| D10Jhu46 | 166J15.SP6 | 139 | F-5′CTCTACCGCAACTCTCAGCC | R-5′TTGGGGTTTAAGTCTGCCAC | Prog 10 at 57 | AF167494 |
| D10Jhu47 | 340M5.SP6 | 348 | F-5′ACTGGGACCACCAACTGAAG | R-5′CCAGAAAGAGCCTCACGAAC | Prog 45 | AF167513 |
| D10Jhu48 | 578K13.SP6 | 281 | F-5′AGATGCATACTGCATGGGTG | R-5′AGTCCCTGCCGATGACATAC | Prog 10 | AF167506 |
| D10Jhu49 | 112I15.T7 | 266 | F-5′AATGCCAATCAAACCTGAGC | R-5′TTTGCCCCTTCAATTACAGC | Prog 1 | AF167490 |
| D10Jhu50 | 340M5.T7 | 331 | F-5′GTAGGGGTGCCAGTGTCATT | R-5′CCCTTTCCCCCACAATCTAT | Prog 10 | AF167514 |
| D10Jhu51 | 335G5.SP6 | 266 | F-5′CAGAGAGAATTCCCCCATCA | R-5′AACTCCCAACCAACACCAAG | Prog 45 | AF167512 |
| D10Jhu52 | 429K7.SP6 | 185 | F-5′AGTCCTGGTCACCAACAAGG | R-5′AGAAATGGGATGCTGTGACC | Prog 45 | AF167523 |
| D10Jhu53 | 118N22.SP6 | 209 | F-5′GTAGGGGAGACCTTTGGGAC | R-5′AGACGATGTCCGGTGGTAAG | Prog 45 | AF167491 |
| D10Jhu54 | 118N22.T7 | 258 | F-5′ACGTAGGTGCCGAGTTCATC | R-5′GAAACCAGGCTGACTGAAGGC | Prog 45 | AF167492 |
| D10Jhu55 | 364C8.SP6 | 103 | F-5′CCAGTTCTCCAAACCTCCAG | R-5′CAGTCAGGCTCACAGAGCAG | Prog 30 | AF167516 |
| D10Jhu56 | 364C8.T7 | 230 | F-5′CCGTGCTAAGAACTACCATGC | R-5′CCCAAACACACACCTCACAC | Prog 1 at 60 | AF167517 |
| D10Jhu57 | 333P4.T7 | 258 | F-5′TTGTCATCTGCCTCTACCCC | R-5′AAGTGCGTAAGCCAGAAAGC | Prog 45 | AF167511 |
| D10Jhu58 | 52K18.SP6 | 187 | F-5′TGCCTATACCACTGTGCCTG | R-5′AGCCGTGTTTTTCCTGTGTC | Prog 45 | AF167503 |
| D10Jhu59 | 52K18.T7 | 429 | F-5′AGCCTGCTTCTTGGTGTCTC | R-5′CATGCTATGTCTGTCCGTGG | Prog 45 | AF167504 |
| D10Jhu60 | 644M8.T7 | 275 | F-5′CTAAGCCTGCATCTTGGGTC | R-5′TCATCAGACCTGGAGCAGTG | Prog 30 | AF167531 |
| D10Jhu61 | 644M8.SP6 | 213 | F-5′TAATGCTCTTGAACCCCTCG | R-5′CTCACAGGGGTCCTGAAAAG | Prog 30 | AF167530 |
| D10Jhu63 | 424J13.T7 | 381 | F-5′AGAACAGAGAGCCAACAGCC | R-5′CTCGAAGACAGTCGTGATGC | Prog 45 | AF167521 |
| D10Jhu64e | 536F4.T7 | 222 | F-5′CGGCATACAATGTCAAGTGG | R-5′TTGTTCCCTTGATCCTCCTG | Prog 10 | AF167515 |
| D10Jhu65 | 424J13.SP6 | 392 | F-5′ATCGGTGGCCGTATAGTCGT | R-5′CACTCTTGGGAAGGAAGCTG | Prog 30 | AF167522 |
| D10Jhu66 | 566H1.T7 | 288 | F-5′CATGATGGTTGCTTGTCCTG | R-5′TGGGGTCTGACTGAAGAACC | Prog 30 | AF167505 |
| D10Jhu67 | 481N4.SP6 | 441 | F-5′AGGAAGGGAAGGAGAAGCAG | R-5′CATCCTGTAGAAAGCGAGCC | Prog 10 | AF167525 |
| D10Jhu68 | 481N4.T7 | 207 | F-5′GTTGCTGACTCTCCTCCCTG | R-5′TAGAGAAGGTTCTGCCTGCC | Prog 45 | AF167526 |
| D10Jhu69 | 12N23.SP6 | 154 | F-5′GGTTCTGCCTGGGAATCTC | R-5′TCCAACATCCTTTTTCTCGG | Prog 10 at 57 | AF167493 |
| D10Jhu70 | 387J11.SP6 | 297 | F-5′GGAAATCTGGAGAGACGCAG | R-5′TGAATGTGGCTGAGTCGAAG | Prog 10 at 57 | AF167519 |
| D10Jhu71e | 12N23.T7 | 233 | F-5′CTTTTCCAGCCCTCACTCAG | R-5′AAAGGCAACATCCCACTCTG | Prog 10 at 57 | AF167537 |
| D10Jhu72 | 387J11.T7 | 228 | F-5′GGGAGGAAGGAGGCAGATAG | R-5′CGGGACTTGCTGTTTTAAGC | Prog 10 at 57 | AF167520 |
| D10Jhu78 | 455H18.T7 | 183 | F-5′CCTCAGGCTGCTCTGACTCT | R-5′TGTCTGGCATCAACAGCTTC | Prog 45 | AF167535 |
| D10Jhu79 | 58L3.T7 | 107 | F-5′TCCATCTTTGAGACATGGGAG | R-5′CATAGACTGGGCCTTAGGGA | Prog 42 | AF167536 |
a
Prog. 1 (94°C × 30"/55°C × 30"/72°C × 1′) 35 cycles. Prog. 10: As Prog. 1 but 45 cycles. Prog. 30: As Prog. 1 but 57°C annealing. Prog. 42: As Prog. 1 but 58°C annealing and 45 cycles. Prog. 45: Touchdown 72°C to 58°C in 2°C steps–5 cycles each.