Skip to main content
. 2011 Jul 1;15(1):1–17. doi: 10.1089/ars.2010.3560

FIG. 1.

FIG. 1.

Expression and activity of members of the Akt kinase family. (A) Reverse transcription–polymerase chain reaction to detect the mRNA levels for Akt1, Akt2, and Akt3 in the retina, lens epithelia, lens fiber, and cornea of the mouse eye. As an internal control, a β-actin DNA band of 242-bp was also amplified. The primers used in the present studies are listed in Table 1. The primers for Akt1, Akt2, Akt3, and β-actin were added to the reactions at the same time. (B) Quantitative results of the mRNA expression levels for Akt1, Akt2, and Akt3 in the retina, lens epithelia, lens fiber, and cornea of the mouse eye. Note that Akt1 is highly expressed in all four ocular tissues. (C) Western blot analysis of the protein levels of Akt1, Akt2, and Akt3 in the retina, lens epithelia, lens fiber, and cornea of the mouse eye (see Materials and Methods section for the details). (D) Quantitative results of the protein expression levels of Akt1, Akt2, and Akt3 in the retina, lens epithelia, lens fiber, and cornea of the mouse eye. (E) Western blot analysis of total Akt1 and Akt1 phosphorylation status at T450, T308, and S473 in the retina, lens epithelia, lens fiber, and cornea of the mouse eye. (F) Quantitative results of total Akt1 and Akt1 phosphorylation status at T450, T308, and S473 in the retina, lens epithelia, lens fiber, and cornea of the mouse eye. Data were analyzed with the Student-Newman-Keuls (SNK) test.