Figure 2.

Megarestriction map and YAC contig of the region containing the loci h49 and j18. (A) Megarestriction map of the region spanning the loci h49 and j18. As described in legend of Figure 1, T. cruzi chromosomal DNA and isolated chromosomal bands XVI and XVII were digested with different restriction enzymes, separated by conventional agarose gel electrophoresis or by PFGE, blotted into nylon membrane, and hybridized with probes H49 and JL8. Restriction enzymes: S, SfI; N, NotI; E, EcoRI; B, BamHI; P, PacI. Red and green boxes indicate the location of genes h49 and jl8, respectively. To facilitate comprehension, the multiple HindIII restriction sites within the repetitive region of gene h49, and HaeIII restriction sites within the repetitive region of gene jl8 were not represented. (B) Results of YAC contiging based on marker content of clones. A subset of 8 YACs, 2 cosmids, and 3 λ phages is shown. Marker ascertainment was carried out by PCR and dot blot hybridization. The exact order of markers has not been established, and the intermarker distances are not to scale. There are several discrepancies in the marker content of the map: markers SRE, gp85, gp90, mucin, and B11 are not contained in several YACs. Cosmids and λ phages containing genes h49 and jl8 are shown below the YACs. The YAC ends (L, left end; R, right end) were determined by inverse PCR (Materials and Methods).