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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: Dev Cell. 2009 Dec;17(6):836–848. doi: 10.1016/j.devcel.2009.10.011

Figure 2. RP58 Expression in Mouse Limb Bud and in C2C12 Culture.

Figure 2

(A) Expression patterns of muscle-related transcription factors (Pax3, Myf5, MyoD, and Myog) and RP58 in mouse limb bud at E9.5, 10.5, and 11.5, as determined by WISH. At the bottom are sections of an E10.5 (Left) or E11.5 (Right) embryo showing RP58 expression. Abbreviations: m, myotome; drg, dorsal root ganglia; nt, neural tube; dmm, dorsal muscle mass; vmm, ventral muscle mass; fl, forelimb. Bar, 500 μm.

(B) Real-time PCR analysis of RP58 and myogenesis-associated genes in C2C12 cells cultured in GM or DM for 0, 2, or 4 days (d). Error bars, SEM (n = 3).

(C) Western blotting for RP58 during C2C12 cell myogenic stages.