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. Author manuscript; available in PMC: 2011 Jun 7.
Published in final edited form as: Dev Cell. 2009 Dec;17(6):836–848. doi: 10.1016/j.devcel.2009.10.011

Figure 3. RP58 Knockdown Inhibits Myogenesis.

Figure 3

(A) Western blotting for RP58 in C2C12 lines stably expressing RP58 shRNA (shRP58) or control shRNA (Ctl) on differentiation day 2.

(B) Immunocytochemistry for MyHC and DAPI staining in C2C12 lines stably expressing RP58 shRNA (shRP58) or control shRNA (Ctl) on differentiation day 4. Bar, 200 μm.

(C) Percentage of MyHC-positive nuclei in RP58 knockdown (shRP58) or control (Ctl) C2C12 cells of (B) in three independent fields. Error bars, SD (n = 3).

(D) H&E staining of hindlimb muscles from WT and RP58−/− mice (E18.5). Higher magnifications of boxed regions in left panels are shown in right panels. Arrowheads indicate an abnormal population of mononucleate cells in RP58−/− skeletal muscle tissues. Bar, 50 μm.

(E) Haematoxylin and eosin staining of diaphragm from WT and RP58−/− mice (E18.5, arrow). Lu, lung; Li, liver. Bars, 50 μm.