Table 2.

Description of the three analyses and their objectives

Analysis	Data	Objective
day0	Gene expression data from the tested brain area of Day0 cows and their average heat scores	To identify genes of which the expression in the tested brain area at the start of estrus was associated with estrous behavior
day12	Gene expression data from the tested brain area of Day12 cows and their average heat scores	To identify genes of which the expression in the tested brain area around mid cycle (diestrus) was associated with estrous behavior
day0 + day12	Gene expression data from the tested brain area of Day0 and Day12 cows and their average heat scores	To identify genes of which the expression in the tested brain area was associated with estrous behavior regardless of the phase of estrous cycle