Table 1. Characteristics of loci used in this study for Glossina fuscipes fuscipes.
Locus | Repeat motif | Primer (5′-3′) | Ta (°C) | PICd | Chromosomal inheritance |
A03 | (CA)12 | F: AGCCGCTTTAAGTTTGTTGC | 54 | 0.639 | autosomal |
R: GTTTGTTCGGTGGGCAATAC | |||||
A06a | (AC)10 | F: TCCCGTCACAGACGTTACAATCAAAGGTGGGTCTATC | 50 | 0.211 | X-linked |
R: CCGAACGAATACAGGTAAAG | |||||
A09a | (TG)6TA(TG)6TA(TG)6 | F: AACTGTTTCTTCAACAAAAATCCA | 54 | 0.099 | X-linked |
R: GAAAATCGCGCATATTGCTA | |||||
A112a | (CA)8 | F: TCCCAGTCACGACGTCGTTTCCTCTTCACCTCCAC | 56 | 0.000 | X-linked |
R: CGGGCTGTCTTCTTTTGG | |||||
B03 | (CT)18 | F: AGTCCGGGGATTTATTGACC | 58 | 0.416 | X-linked |
R: TGGTTGGTACTGATGCGAAA | |||||
B05b | (GA)12 | F: CGCGCTTAGCTAGGAAACTC | 58 | 0.364 | X-linked |
R: AACGATTTGCTGTCCTCGAT | |||||
B06 | (GA)18TA(GA)2 | F: TCCCAGTCACGACGTAGTGCCAATGAAGAGAGTGTC | 50 | 0.844 | autosomal |
R: CCAACTGTTTAGGGCTGTTC | |||||
B11 | (GA)28 | F: TTAGAGCCAGTGCCAATGAA | 54 | 0.866 | autosomal |
R: TCAGTGAAGACATATTCGCATGTA | |||||
B109 | (GA)9 | F: CCGAGAGAGTAGCGGAGAGA | 58 | 0.228 | X-linked |
R: CCGGCTATGCCCACTTAATA | |||||
C7c | (TGA)18 | F: GAATTTTAACAAATTGGACTTACAG | 54 | 0.696 | autosomal |
R: CCAGGTTAAAACCAGTAACTTCC | |||||
C104 | (TGA)7TGG(TGA)6 | F: TGTGAGTACTCGGGGATCTCT | 52 | 0.000 | autosomal |
R: GGCAGCAACAAGTTTCCATT | |||||
C107a | (TCA)12 | F: TCCCAGTCACGACGTCGCGGCACCTGTTAGTTAGT | 53 | 0.603 | X-linked |
R: GGCACGTGAACTAATGCAAA | |||||
D3 | (CAG)9 | F: CTTTAATGGCTTCGCAGGAG | 58 | 0.572 | X-linked |
R: TGCGCATTATTTGATGTTGC | |||||
D05 | (GCA)10 | F: TTGGATTAGCAGCACGAATG | 58 | 0.405 | autosomal |
R: GGGAATTGGAATTGTGAGGA | |||||
D06a | (GCT)9 | F: TAACGGGGAGCTAAAAGAAG | 53 | 0.064 | X-linked |
R: AAATCATCAGCAGCATCATC | |||||
D12 | (CTG)8 | F: ATTCCCATTGCTGGTTGATG | 56 | 0.118 | X-linked |
R: ACGGGTTAGCAAATGAAACG | |||||
D101 | (TGC)8 | F: CTCATGCGTCTGCCTTTACA | 58 | 0.639 | autosomal |
R:AGGAGCAATGATGTGTTGGA | |||||
D103 | (GCTGAT)7 | F: GCGGTGTTGCTAGTGGTGTA | 53 | 0.434 | X-linked |
R: CCAAGACCATTAACCCATGC | |||||
D109a | (AGC)9 | F: TCCCAGTCACGACGTATCTGCCAATGACATGAATATC | 59 | 0.107 | X-linked |
R: CAGTTGGTGTCCGTGTGT |