Figure 7. PIN3- and PIN7-GFP localization in dark-acclimated seedlings.

(A) Functional PIN3:PIN3-GFP fluorescence in hypocotyls of seedlings exposed to blue light (BL, 1 µmol m⁻² s⁻¹) for 6 h or continued darkness. A blue-light-treated seedling is shown on the right and white arrows indicate a decrease in PIN3:PIN3-GFP signal. Scale bar  = 200 µm. (B) Functional PIN7:PIN7-GFP fluorescence in hypocotyls of seedlings exposed to blue light (1 µmol m⁻² s⁻¹) for 12 h or continued darkness. Scale bar  = 20 µm. Heat map representations of GFP signals are shown on the right. In each case data are representative of n>20 seedlings.