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. 2011 Jun 7;6(6):e20567. doi: 10.1371/journal.pone.0020567

Figure 1. Imipramine specifically activates TrkB receptors in the mouse brain.

Figure 1

A) The ability of imipramine (30 mg/kg, i.p., 30 min; n = 6/group) to induce rapid TrkB phosphorylation in the hippocampus and medial prefrontal cortex of TrkBF616A mutant mice is abolished with 1NaPP1 pretreatment (25 µM for 1-week in drinking water+83 ng/g co-injection with imipramine). B) A representative blot showing TrkB kinase dependent action of imipramine-induced phosphorylation of TrkB (Y816) and the ∼105 kDa protein. C) A representative blot showing imipramine-induced TrkB phosphorylation in flag-precipitated pool of protein from the brains of mice over-expressing flag-tagged catalytic TrkB receptors. Data is presented as percentage of control/saline ± standard error of mean (SEM). *<0.05, **<0.01; two-way ANOVA with Newmann-Keuls post hoc test.