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. 2011 Jun 7;6(6):e20567. doi: 10.1371/journal.pone.0020567

Figure 4. Antidepressant drugs amitriptyline and imipramine do not regulate TrkB phosphorylation in primary neurons.

Figure 4

A) Whereas BDNF (20 ng/ml; 15 min) robustly increases the phosphorylation of TrkB (Y816) in E18 rat cortical and hippocampal neurons (14 DIV), amitriptyline (left & middle; 0.5 µM, 5 µM; 15 min) and imipramine (0.5 µM, 5 µM; 50 µM; 15 min) produces no change on TrkB phosphorylation. Representative blot of triplicate data. B) Imipramine pre-treatment (4, 12, 40 µM; 15 min) did not facilitate BDNF-induced (5 ng/ml; 15 min) TrkB phosphorylation in E18 rat cortical neurons as measured with phospho-TrkB ELISA. n = 4/group. C) Imipramine pre-treatment (4, 12, 40 µM; 15 min) did not regulate TrkB phosphorylation in its own or in combination with depolarization stimuli (50 mM KCl; 15 min) as measured with phospho-TrkB ELISA. n = 4/group. Data is presented as percentage of control ± standard error of mean (SEM). *<0.05; one-way ANOVA with Newmann-Keuls post hoc test.