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. 2011 Jun 7;6(6):e20567. doi: 10.1371/journal.pone.0020567

Figure 5. Monoamines and monoamine reuptake in TrkB activation in vitro and in vivo.

Figure 5

A) Serotonin selective reuptake inhibitor fluoxetine produced essentially similar changes on hippocampal TrkB phosphorylation in the brains of wild-type and serotonin transporter KO mice, sert −/−. n = 3–5/group. B) Whereas the ability of serotonergic antidepressant citalopram (20 mg/kg, i.p., 60 min) and norepinephrinergic antidepressant reboxetine (20 mg/kg, i.p., 30 min) to induce full-length TrkB receptor phosphorylation (Y816) in mice depleted of serotonin (with pCPA) or norepinephrine (with DSP-4) are reduced, ∼105 kDa protein is heavily phosphorylated by both drugs in the hippocampi of these mice. Representative blots. n = 6–7/group. C) Norepinephrine (NE; 10 nM-10 µM; 15 min) and serotonin (5-HT; 10 nM-10 µM; 15 min) produced no changes on TrkB phosphorylation in BDNF-responsive E18 rat cortical neurons (15DIV) as measured with phospho-TrkB ELISA. n = 4/group. Data is presented as percentage of control ± standard error of mean (SEM). *<0.05; two-way ANOVA with Newmann-Keuls post hoc test.