Figure 2. In vitro characterization of clonally expanded h-ASCs for mesenchymal markers.

Representative immunostaining of cytoskeletal protein vimentin (A), alpha smooth muscle actin (B), nestin (C), fibronectin (D) and cell proliferation marker Ki-67 (E) with immunofluoresence staining. Negative control staining with fluorescence conjugated secondary antibodies shown (F). Cell nuclei were stained with DAPI. Scale bar, 20 µm. h-ASCs exhibited competence to differentiate into adipogenic, osteogenic and chondrogenic lineages in-vitro upon specific induction as confirmed by Oil red O (G), Saffranin-O (H) and Alizarin-red (I) staining respectively.