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. 2011 Jun 7;6(6):e20725. doi: 10.1371/journal.pone.0020725

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Shetty et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A. Analysis of lipopolysaccaride (LPS) by tricine-SDS-PAGE and silver staining. Samples of LPS were extracted from: purified nanopods (Lane 2), Delftia sp. Cs1-4 cells grown on phenanthrene (Lane 3) and Delftia sp. Cs1-4 cells grown on pyruvate (Lane 4). Lane 1 is an LPS standard (Salmonella typhimurium). The arrow indicates a dense band of LPS present in whole cell preparations but absent from nanopods. All samples were loaded at the same dry weight (200 µg). Panel B. SDS-PAGE Protein profiles of nanopods (Lane 1) and the outer membrane (OM) of phenanthrene-grown Delftia sp. Cs1-4 cells (Lane 2). Proteins identified in gel slices from the OM sample are (locus in Delftia sp. Cs1-4): 1. NpdA (DelCs14_2799), 2. Fiu-like TonB-dependent siderophore receptor (DelCs14_0908), 3. TonB-dependent siderophore receptor (DelCs14_5618) 4. Protein with domain of unknown function 1302 (DelCs14_4425), 5. RND efflux system, outer membrane lipoprotein (DelCs14_5845) 6. 4. Type II L-Asparaginase, 7. OmpC-like protein (DelCs14_0125), 8. Omp32 (DelCs14_0124). 9. OmpA-like protein (DelCs14_3211).