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Mice were immunized s.c. on days 0 and 21, and bled on day 42. Neutralizing antibody titers and HA-specific IgG subclasses were measured by HAI test (A, n = 10) and ELISA (B, (n = 5), respectively, and expressed as GMTs ±95%CI. **, p<0.01 in 2-way ANOVA/Bonferroni test.
