Table 2. HAI titers and survival rates of immunized mice.
| Vaccines | Dose (µg) | Experiment 1 | Experiment 2 | ||
| GMT (SP) | Survival (%) | GMT (SP) | Survival (%) | ||
| STF2.HA1 | 3 | 116 (93) | 100*** | 91 (64) | 100*** |
| 0.3 | 17 (33) | 100*** | 19c (44) | 100*** | |
| 0.03 | 5 (0) | 20 | 6d (0) | 90** | |
| STF2R3.HA1 | 3 | 139 (93) | 100*** | 300 (100) | 100*** |
| 0.3 | 30a , b (60) | 100*** | 53 (67) | 100*** | |
| 0.03 | 6 (0) | 60* | 10 (7) | 100*** | |
| STF2R3.2xHA1 | 3 | 211 (93) | 100*** | 234 (100) | 100*** |
| 0.3 | 88 (93) | 100*** | 153 (87) | 100*** | |
| 0.03 | 10 (20) | 90*** , e | 22 (33) | 100*** | |
| F147 | N/A | 5 (0) | 0 | 6 (0) | 10 |
Mice were immunized s.c. on days 0 and 21, bled on day 35, and challenged I.N. with 500 TCID50 of mouse adapted A/California/04/2009 on day 42. Infected mice were monitored daily (n = 10) for mortality for 21 days. SP: seroprotective titer, mice% with ≥40 HAI titers.
Two-way ANOVA/Boferroni tests for HAI data (n = 15):
a
, p<0.01 (**) vs STF2.HA1;
b
, p<0.001 (***) vs STF2R3.2xHA1;
c
, p<0.001 (***) vs STF2R3.2xHA1;
d
, p<0.05 vs STF2R3.2xHA1.
Fisher's exact test for survival data (n = 10):
*
, p<0.05;
**
, p<0.01;
***
, p<0.001;
e
, p<0.05 (*) vs STF2.HA1 at the same dose (0.03 µg/mouse).