|
Inflammatory factor related
|
|
| Interleukin-6 (IL-6) |
TTCCAGCCAGTTGCCTTCTT
TGTTGTGGGTGGTATCCTCTGT
|
| Tumor necrosis factor-α (TNF-α) |
ACGGAAAGCATGATCCGAGAT
GCCACGAGCAGGAATGAGAA
|
| Cyclooxygenase-2 (COX-2) |
GGAGGAGAAGTGGGGTTTAGGA
TGCGTTGATGGTGGCTGTC
|
|
Stromal-vascular fraction mark genes
|
|
| Macrophage expressed gene 1 (MEPG-1) |
ATTCAGGGCTTATTGGTGCG
GACTGTGCATTTGTCATTGGGT
|
| Cluster of differentiation 68 (CD68) |
ATGCCACAGTTTCTCCCACC
TGTAGTTTCCAAGAGCCCCAGT
|
| Macrophage migration inhibitory factor (MIF) |
CGGACCGGGTCTACATCAACTA
GCAGCAAGACTCGAAGAACAGC
|
| F4/80 |
TGGAATGCATAATCGCTGCT
CAAGGAGGGCAGAGTTGATC
|
| Cluster of differentiation 64 (CD64) |
CACCGTGAAAGAGCTATTTGC
AAGTAAAGCCGTAAGCCAGG
|
| Adipose differentiation related protein (ADRP, or Perilipin-2) |
TGGGTGGAGTGGAAGAGAAT
ATGTGACTCGATGTGCTCAG
|
| Leucine-rich alpha-2-glycoprotein 1 (LRG1) |
CATCAAGGGAGAACCCTGTT
CCGACTGCAGTATCAAGCAT
|
| Myeloperoxidase (MPO) |
TACGGGATGGCGATAGGTTT
TGTTTACTGAACACACCCGG
|
|
Adipose tissue lipolysis related
|
|
| Adipose triglyceride lipase (ATGL) |
TGGATGGCGGCATTTCA
GTTCGTGGATGTTGGTGGAG
|
| Hormone sensitive lipase (HSL) |
AGCGCCTATTCAGGGACAGA
CCAGGAAGGAGTTGAGCCAT
|
| Perilipin-1 |
GTACACTATGTCCCGCTTCC
CCACCTCTGCTGGAGGATTA
|
|
Adipokine genes
|
|
| Adiponectin |
GGGACAACAATGGACTCTATGC
GTTCTTTGATTCTCGGGGCTA
|
| Lipocalin-2 |
GACAACTGAACAGACGGTGAGC
CTGGCAACAGGAAAGATGGA
|
|
Hepatocyte apoptosis related
|
|
| Caspase-3 |
TGACGACAGGGTGCTACGAT
TGAGGCTGCTGCATAATCG
|
| Caspase-8 |
GAAGAACTGGCTGCCCTCAA
GGACAAATTGTCTTCCTCCAAC
|
| B-cell lymphoma 2 (BCL-2) |
TGTGGCCTTCTTTGAGTTCG
ATCCCAGCCTCCGTTATCCT
|
|
Hepatocyte insulin-sensitive glycogen synthesis related
|
|
| Insulin receptor substrate 1 (IRS-1) |
TCTACACCCGAGACGAACAC
GGCCTTTGCCCGATTAT
|
| Glucose transporter 2 (GLUT-2) |
ACACCAGCACATACGACACCA
CAAAGAACGAGGCGACCATT
|
| Glycogen synthase 2 (GYS-2) |
ACTCCAAACGGCTTGAACG
GCCATAGAAATGACCTCGAACA
|
|
Reference genes
|
|
| β-actin |
AGGGAAATCGTGCGTGAC
CGCTCATTGCCGATAGTG
|
| Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) |
GGCAAGTTCAACGGCACAGT
CGCCAGTAGACTCCACGACATA
|
| TATAA-box binding protein (TBP) |
CCACCGTGAATCTTGGCTGTA
ACGCAGTTGTTCGTGGCTCT
|
