Figure 9. The substitution of V₇₅H₇₆ by T₇₅E₇₆ in TF_1–102 reduced the interaction between the fragment and MAb_25C11 and MAb_20B9.

The AapBrpt1.5 fragment, TF_1–102, with the substitution of V₇₅H₇₆ by T₇₅E₇₆ was established using site-directed mutagenesis. The interactions between the mutated fragment and MAb_25C11 and MAb_20B9 were detected using immunoprecipitation. “TF_1–102V₇₅H₇₆”: wild type, “TF_1–102T₇₅E₇₆”: mutant, “IgG H”: IgG heave chain.